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yfp-pop1 expression vector  (Addgene inc)


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    Addgene inc yfp-pop1 expression vector
    MOB3C interacts with catalytically active RNase P complex. A , coimmunoprecipitation assay for <t>YFP-tagged</t> <t>POP1</t> and RPP30 with FLAG-tagged MOB3C in HeLa cells. B , schematic outline for the affinity purification–mass spectrometry (AP–MS) with DSP crosslinking (CL). A Western blot analysis validating the used cell lines in AP–MS is shown. C , Venn diagram showing the commonly defined proteins in the BioID and crosslinked AP–MS datasets. The table displays the average spectral counts of each RNase P subunit defined in the AP–MS dataset. D , a proximity network of the RNase P protein subunits defined in both the MOB3C BioID and AP–MS datasets in reference to BioGrid (see the section). E , pre-tRNA cleavage assay of the pull-down experiments using GST-MOB1A or GST-MOB3C. Time-course reactions showing RNase P activity when GST-MOB3C, but not GST-MOB1A, was used. +: a positive control of pre-tRNA Arg cleaved by recombinant Escheichia coli RNase P; –: a negative control of pre-tRNA Arg without enzyme; I: input. F , SDS-PAGE analysis confirms the presence of GST-MOB3C and GST-MOB1A post-pulldown. DSP, dithiobis (succinimidyl propionate); EV, empty vector; GST, glutathione- S -transferase; MOB, monopolar spindle-one-binder.
    Yfp Pop1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yfp-pop1+expression+vector/pmc10480535-213-0-16?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    yfp-pop1 expression vector - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Mapping the MOB proteins’ proximity network reveals a unique interaction between human MOB3C and the RNase P complex"

    Article Title: Mapping the MOB proteins’ proximity network reveals a unique interaction between human MOB3C and the RNase P complex

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2023.105123

    MOB3C interacts with catalytically active RNase P complex. A , coimmunoprecipitation assay for YFP-tagged POP1 and RPP30 with FLAG-tagged MOB3C in HeLa cells. B , schematic outline for the affinity purification–mass spectrometry (AP–MS) with DSP crosslinking (CL). A Western blot analysis validating the used cell lines in AP–MS is shown. C , Venn diagram showing the commonly defined proteins in the BioID and crosslinked AP–MS datasets. The table displays the average spectral counts of each RNase P subunit defined in the AP–MS dataset. D , a proximity network of the RNase P protein subunits defined in both the MOB3C BioID and AP–MS datasets in reference to BioGrid (see the section). E , pre-tRNA cleavage assay of the pull-down experiments using GST-MOB1A or GST-MOB3C. Time-course reactions showing RNase P activity when GST-MOB3C, but not GST-MOB1A, was used. +: a positive control of pre-tRNA Arg cleaved by recombinant Escheichia coli RNase P; –: a negative control of pre-tRNA Arg without enzyme; I: input. F , SDS-PAGE analysis confirms the presence of GST-MOB3C and GST-MOB1A post-pulldown. DSP, dithiobis (succinimidyl propionate); EV, empty vector; GST, glutathione- S -transferase; MOB, monopolar spindle-one-binder.
    Figure Legend Snippet: MOB3C interacts with catalytically active RNase P complex. A , coimmunoprecipitation assay for YFP-tagged POP1 and RPP30 with FLAG-tagged MOB3C in HeLa cells. B , schematic outline for the affinity purification–mass spectrometry (AP–MS) with DSP crosslinking (CL). A Western blot analysis validating the used cell lines in AP–MS is shown. C , Venn diagram showing the commonly defined proteins in the BioID and crosslinked AP–MS datasets. The table displays the average spectral counts of each RNase P subunit defined in the AP–MS dataset. D , a proximity network of the RNase P protein subunits defined in both the MOB3C BioID and AP–MS datasets in reference to BioGrid (see the section). E , pre-tRNA cleavage assay of the pull-down experiments using GST-MOB1A or GST-MOB3C. Time-course reactions showing RNase P activity when GST-MOB3C, but not GST-MOB1A, was used. +: a positive control of pre-tRNA Arg cleaved by recombinant Escheichia coli RNase P; –: a negative control of pre-tRNA Arg without enzyme; I: input. F , SDS-PAGE analysis confirms the presence of GST-MOB3C and GST-MOB1A post-pulldown. DSP, dithiobis (succinimidyl propionate); EV, empty vector; GST, glutathione- S -transferase; MOB, monopolar spindle-one-binder.

    Techniques Used: Co-Immunoprecipitation Assay, Affinity Purification, Mass Spectrometry, Western Blot, Cleavage Assay, Activity Assay, Positive Control, Recombinant, Negative Control, SDS Page, Plasmid Preparation



    Similar Products

    yfp-pop1 expression vector  (Addgene inc)
    90
    Addgene inc yfp-pop1 expression vector
    MOB3C interacts with catalytically active RNase P complex. A , coimmunoprecipitation assay for <t>YFP-tagged</t> <t>POP1</t> and RPP30 with FLAG-tagged MOB3C in HeLa cells. B , schematic outline for the affinity purification–mass spectrometry (AP–MS) with DSP crosslinking (CL). A Western blot analysis validating the used cell lines in AP–MS is shown. C , Venn diagram showing the commonly defined proteins in the BioID and crosslinked AP–MS datasets. The table displays the average spectral counts of each RNase P subunit defined in the AP–MS dataset. D , a proximity network of the RNase P protein subunits defined in both the MOB3C BioID and AP–MS datasets in reference to BioGrid (see the section). E , pre-tRNA cleavage assay of the pull-down experiments using GST-MOB1A or GST-MOB3C. Time-course reactions showing RNase P activity when GST-MOB3C, but not GST-MOB1A, was used. +: a positive control of pre-tRNA Arg cleaved by recombinant Escheichia coli RNase P; –: a negative control of pre-tRNA Arg without enzyme; I: input. F , SDS-PAGE analysis confirms the presence of GST-MOB3C and GST-MOB1A post-pulldown. DSP, dithiobis (succinimidyl propionate); EV, empty vector; GST, glutathione- S -transferase; MOB, monopolar spindle-one-binder.
    Yfp Pop1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yfp-pop1+expression+vector/pmc10480535-213-0-16?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    yfp-pop1 expression vector - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    MOB3C interacts with catalytically active RNase P complex. A , coimmunoprecipitation assay for YFP-tagged POP1 and RPP30 with FLAG-tagged MOB3C in HeLa cells. B , schematic outline for the affinity purification–mass spectrometry (AP–MS) with DSP crosslinking (CL). A Western blot analysis validating the used cell lines in AP–MS is shown. C , Venn diagram showing the commonly defined proteins in the BioID and crosslinked AP–MS datasets. The table displays the average spectral counts of each RNase P subunit defined in the AP–MS dataset. D , a proximity network of the RNase P protein subunits defined in both the MOB3C BioID and AP–MS datasets in reference to BioGrid (see the section). E , pre-tRNA cleavage assay of the pull-down experiments using GST-MOB1A or GST-MOB3C. Time-course reactions showing RNase P activity when GST-MOB3C, but not GST-MOB1A, was used. +: a positive control of pre-tRNA Arg cleaved by recombinant Escheichia coli RNase P; –: a negative control of pre-tRNA Arg without enzyme; I: input. F , SDS-PAGE analysis confirms the presence of GST-MOB3C and GST-MOB1A post-pulldown. DSP, dithiobis (succinimidyl propionate); EV, empty vector; GST, glutathione- S -transferase; MOB, monopolar spindle-one-binder.

    Journal: The Journal of Biological Chemistry

    Article Title: Mapping the MOB proteins’ proximity network reveals a unique interaction between human MOB3C and the RNase P complex

    doi: 10.1016/j.jbc.2023.105123

    Figure Lengend Snippet: MOB3C interacts with catalytically active RNase P complex. A , coimmunoprecipitation assay for YFP-tagged POP1 and RPP30 with FLAG-tagged MOB3C in HeLa cells. B , schematic outline for the affinity purification–mass spectrometry (AP–MS) with DSP crosslinking (CL). A Western blot analysis validating the used cell lines in AP–MS is shown. C , Venn diagram showing the commonly defined proteins in the BioID and crosslinked AP–MS datasets. The table displays the average spectral counts of each RNase P subunit defined in the AP–MS dataset. D , a proximity network of the RNase P protein subunits defined in both the MOB3C BioID and AP–MS datasets in reference to BioGrid (see the section). E , pre-tRNA cleavage assay of the pull-down experiments using GST-MOB1A or GST-MOB3C. Time-course reactions showing RNase P activity when GST-MOB3C, but not GST-MOB1A, was used. +: a positive control of pre-tRNA Arg cleaved by recombinant Escheichia coli RNase P; –: a negative control of pre-tRNA Arg without enzyme; I: input. F , SDS-PAGE analysis confirms the presence of GST-MOB3C and GST-MOB1A post-pulldown. DSP, dithiobis (succinimidyl propionate); EV, empty vector; GST, glutathione- S -transferase; MOB, monopolar spindle-one-binder.

    Article Snippet: YFP-POP1 expression vector was generated by amplifying the POP1 cDNA from pCMVh-POP1-3xFLAG (gift from Martin Dorf; Addgene #53968) with flanking XhoI and SalI restriction sites and ligating it into the multicloning site of YFP-C1 backbone vector by swabbing out the YFP-RPP30.

    Techniques: Co-Immunoprecipitation Assay, Affinity Purification, Mass Spectrometry, Western Blot, Cleavage Assay, Activity Assay, Positive Control, Recombinant, Negative Control, SDS Page, Plasmid Preparation